YSI 2900D handleiding
Handleiding
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8-19
8.2.18 Simultaneous L-Glutamate and L-Glutamine
Refer to the previous sections on L-Glutamate and L-Glutamine for theoretical and special considerations, then follow the
instructions below to set up your instrument for simultaneous determination.
A Probe
B Probe
Chemistry
Glutamine
Glutamate
System
Buffer
YSI 2357
Calibrator
Std
YSI 2736
YSI 2755
Linearity
Std
YSI 2737
YSI 2756
Membrane
YSI 2735
YSI 2754
Sample
Size
20 µL
Unit of
Conc.
mmol/L
Cal Value
5.00
5.00
End Point
30 sec
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Since the Glutamine Membrane (2735) utilizes a chain of
enzyme reactions and the final reaction is a “glutamate
measurement”, free glutamate in a sample will be measured
at the Glutamine Sensor. By using the information at the
Glutamate Sensor, this free glutamate is automatically
subtracted in the instrument’s software. The algorithm looks
basically like this:
(“Total Glutamate” - “Free Glutamate”) = Glutamine
Total Glutamate is the combined glutamine and glutamate
probe signal. Free Glutamate is the glutamate not derived
from Glutamine reaction.
Special Considerations:
• The ability to select sample size (10 to 50 µL) may help you
optimize your system, especially if very high or very low
concentrations of one or the other of these substrates is a
problem. The optimal probe current for a calibrator is about
10 nA.
• When measuring glutamate and glutamine, the glutamate
and glutamine sensors must be located in the same module.
Bekijk gratis de handleiding van YSI 2900D, stel vragen en lees de antwoorden op veelvoorkomende problemen, of gebruik onze assistent om sneller informatie in de handleiding te vinden of uitleg te krijgen over specifieke functies.
Productinformatie
| Merk | YSI |
| Model | 2900D |
| Categorie | Niet gecategoriseerd |
| Taal | Nederlands |
| Grootte | 50780 MB |
