YSI 2900D handleiding
Handleiding
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8-17
8.2.16 Simultaneous Glucose and Sucrose
Refer to the sections above on Glucose and Sucrose for theoretical and special considerations. Then follow the
instructions below to set up your instrument for simultaneous determination.
A Probe
B Probe
Chemistry
Sucrose
Glucose
System
Buffer
YSI 2357
Calibrator
Std
YSI 2780
YSI 2776
Linearity Std
YSI 2778
YSI 1531
Membrane
YSI 2703
YSI 2365
Sample Size
25 µL
Unit of
Conc.
g/L
Cal Value
5.00
2.50
End Point
30 sec
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Since the Sucrose Membrane (2703) utilizes a chain of
enzyme reactions and the final reaction is a “glucose
measurement”, free glucose in a sample will be measured at
the Sucrose Sensor. By using the information at the Glucose
Sensor, this free glucose is automatically subtracted in the
instrument software.
Sucrose Specifications:
When simultaneously measuring glucose and sucrose, the
sucrose precision and linearity are as follows:
Precision (CV,n=10): 4% or 0.04 g/L, whichever is greater
Linearity: ±5%
Special Considerations:
• When measuring glucose and sucrose, the glucose and
sucrose sensors must be located in the same module.
• The combined total of glucose + sucrose cannot exceed
25 g/L. The glucose concentration cannot exceed 10 g/L
if the combined total is near 25 g/L, since a glucose
concentration that high combined with 15 g/L sucrose will
saturate the Sucrose probe. If either of these conditions
occur, you should dilute your sample to bring it into a
reasonable range.
• The ability to select sample size (10 to 50 µL) may help
you optimize your system, especially if very high or very
low concentrations of one or the other of these
substrates is a problem. The optimal probe current for a
calibrator is about 10 nA. See 4.11 Check Probe
Currents, to learn more about measuring your probe
signal.
• If the glucose concentration in your sample exceeds the
sucrose concentration, the subtraction of a large glucose
“signal” from a smaller sucrose “signal” may result in
significant errors in the sucrose reading. An alternative
method involves using two different, and appropriate
dilutions of the sample to first measure free glucose,
then treat one sample with invertase and analyze for
glucose liberated from the sucrose. Contact YSI
Technical Support or your dealer representative for an
application note explaining this approach in detail.
Bekijk gratis de handleiding van YSI 2900D, stel vragen en lees de antwoorden op veelvoorkomende problemen, of gebruik onze assistent om sneller informatie in de handleiding te vinden of uitleg te krijgen over specifieke functies.
Productinformatie
| Merk | YSI |
| Model | 2900D |
| Categorie | Niet gecategoriseerd |
| Taal | Nederlands |
| Grootte | 50780 MB |
