YSI 2900D handleiding
Handleiding
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8-8
8.2.7 Glycerol
This is a direct reading of glycerol in solution at the enzyme sensor. The enzyme Galactose oxidase is immobilized in the
enzyme membrane.
Glycerol + O
2
Gal Oxidase
H
2
O
2
+ Glycerol Aldehyde Derivative
System Buffer
YSI 2705
Calibrator Std
YSI 7141
Linearity Std
YSI 7142
Membrane
YSI 7140
Membrane
Color
Magenta
Detection
Range
0.75–40.0 g/L
Calibration
Point
25.0 g/L
Linearity Check
Point
40.0 g/L
Sample Size
10 µL
End Point
30 sec
Precision
(CV,n=10)
3% or 0.03 g/L,
whichever is
greater
Linearity
±3% or 0.03 g/L,
whichever is
greater
(0.75 to Cal Point)
±5% or 0.75 g/L,
whichever is
greater
(Above Cal Point to
Range Max)
Typical Working
Life
10 days
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Special Considerations:
• If sample dilution is required, use reagent water.
• The sample should be lactose-free. Lactose and other
galactosides such as galactose, raffinose and stachyose
are substrates for galactose oxidase. They may interfere
by producing artificially high glycerol readings.
• The enzyme membrane integrity test involving the use of
YSI 2363 Potassium Ferrocyanide solution is not
informative since ferrocyanide exists in the 2705 system
buffer.
• Always use fresh YSI 2705 Buffer in an opaque buffer
bottle, such as the YSI 2935, when installing a new YSI
7140 Glycerol Membrane. Do not expose the 2705 Buffer
to light any more than necessary before it is installed in
the 2935 Bottle.
• For maximum performance on the first day the glycerol
membrane is installed, calibrate before every sample.
• If membrane sensitivity drops below 5nA, increase
sample size as required.
• Reduce loss in membrane sensitivity over time by
calibrating glycerol membranes every 2 to 4 hours while
the instrument is idle.
• Decontaminate the 2935 Buffer bottle and cap at least
monthly with an authorized cleaning solution (see
Section 9.1).
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Productinformatie
| Merk | YSI |
| Model | 2900D |
| Categorie | Niet gecategoriseerd |
| Taal | Nederlands |
| Grootte | 50780 MB |
