YSI 2900D handleiding
Handleiding
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8-6
8.2.5 L-Glutamate (L-Glutamic Acid)
This is a direct reading of L-glutamate in solution at the enzyme sensor. The enzyme L-Glutamate Oxidase is immobilized
in the YSI Glutamate Membrane.
L-Glutamate + O
2
Glut Oxidase
H
2
O
2
+ α-Ketoglutarate + NH
3
System Buffer
YSI 2357
Calibrator Std
YSI 2755
Linearity Std
YSI 2756
Membrane
YSI 2754
Membrane
Color
Yellow
Detection
Range
15–1460 mg/L
Calibration
Point
731 mg/L
Linearity Check
Point
1462 mg/L
Sample Size
25 µL
End Point
30 sec
Precision
(CV,n=10)
2% or 8 mg/L,
whichever is
greater
Linearity
±2% or 8 mg/L,
whichever is
greater
(15 to Cal Point)
±5%
(Above Cal Point to
Range Max)
Typical Working
Life
7 days
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Special Considerations:
• If sample dilution is required, use reagent water.
• If you want to measure MSG (monosodium glutamate,
monohydrate) and express the results as mmol/L of
MSG, the calibration parameters listed above are all
appropriate. If you want to express results as a w/v
measurement, such as mg/L MSG, then correction for
the sodium and water components is necessary.
To express results as an MSG value, change the glutamate
units to a w/v expression, and enter one of the following: 936
mg/L, 0.936 g/L, or 0.094 %. Use YSI 2755 Calibrator
Standard (5.00 mmol/L Glutamate) to calibrate the
instrument. Sample results will then reflect MSG
concentration.
Potential Interferences
Tests with equimolar concentrations of other amino acids and
related substances showed little or no interference. On a
scale where Glutamate = 100%, those substances that
produced more than 0.5% response were L-Aspartate (0.7),
L-Tyrosine (1.4), L-Histidine (0.7), and L-Glutamine (1.1).
Glutamate cannot be ruled out as a contaminant in the
Glutamine solution.
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Productinformatie
| Merk | YSI |
| Model | 2900D |
| Categorie | Niet gecategoriseerd |
| Taal | Nederlands |
| Grootte | 50780 MB |
