YSI 2900D handleiding
Handleiding
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8-3
8.2.2 Ethanol
This is a direct reading of Ethanol in solution at the enzyme sensor. The enzyme Alcohol Oxidase is immobilized in the
enzyme membrane.
Ethanol + O
2
Alcohol Oxidase
H
2
O
2
+ Acetaldehyde
System Buffer
YSI 1579
Calibrator Std
YSI 2790
Linearity Std
YSI 2790
Membrane
YSI 2786
Membrane Color
Green
Calibrator
Location
Station 2
(Tube holder)
Detection Range
0.04–3.20 g/L
Calibration
Point
2.00 g/L
Linearity Check
Point
3.20 g/L
Sample Size
15 µL
End Point
45 sec
Precision
(CV,n=10)
2% or 0.02 g/L,
whichever is
greater
Linearity
±2% or 0.02 g/L,
whichever is
greater
(0.04 to Cal Point)
±5%
(Above Cal Point to
Range Max)
Typical Working
Life
5 days
Note: See Appendix B – Concentration
Unit Conversion if concentration unit
conversion is required.
Special Considerations:
• The linear range of ethanol is quite limited. If you are
concerned about linearity, monitor the upper range of
concentration on a regular basis. You may benefit by
preparing and using a calibrator with an ethanol
concentration close to your sample concentration.
• If you prepare your own ethanol calibrator, prepare a
solution with ionic strength. Level sensing depends on a
conductive solution. A 0.1% K
2
EDTA solution as a diluent
acts as both a conductive solution and a preservative.
Normal saline solution (0.9%) is also an acceptable
diluent.
• Your sample should be methanol-free. Methanol can
be a significant interference, since it is a good substrate
for Alcohol Oxidase. Propanol and butanol are very weak
substrates of Alcohol Oxidase and usually do not present
an interference problem.
• Controlling evaporation of ethanol from both sample and
calibrator is important. The use of some type of test tube
cover (eg., prepunctured film) will help. Frequently
replacing the calibrator solution with fresh solution will
also minimize the effects of evaporation on measurement
integrity.
• If the ionic strength of your samples is too low for the
sipper to detect the fluid, turn off sipper fluid detection
and use fixed depth.
Bekijk gratis de handleiding van YSI 2900D, stel vragen en lees de antwoorden op veelvoorkomende problemen, of gebruik onze assistent om sneller informatie in de handleiding te vinden of uitleg te krijgen over specifieke functies.
Productinformatie
| Merk | YSI |
| Model | 2900D |
| Categorie | Niet gecategoriseerd |
| Taal | Nederlands |
| Grootte | 50780 MB |
