Thames & Kosmos Kids First: Biology Lab (V 2.0) handleiding

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Thames & Kosmos

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Handleiding

Microscopy Basics

3. Take the prepared slide of the onion skin.

Scientists refer to the onion skin as the

epidermis of the onion. Position the specimen

as precisely as possible over the center of the

opening in the stage (the aperture) so it is well

illuminated by the light source.

4. Rotate the nosepiece so that the objective lens

with the lowest magnification (4x) is above the

slide. Looking through the eyepiece, use the

fine adjustment knob to lower the objective

lens all the way down and then gradually up

again until the image is sharp.

5. Next try rotating the nosepiece to the two

other magnification levels, using the fine

adjustment knob to sharpen the image each

time.

• If you just see a blurry image, it is because the two lenses (in the eyepiece and the

nosepiece) are not yet at the optimum distance from each other. To get a clear

enlarged image, the distance between the lenses will have to be adjusted (just like with

the “two-water-drop microscope” in the ﬁrst experiment). To do this, slowly (!) turn

the ﬁne adjustment knob while looking through the eyepiece, and you will get a clear

image. Don’t get frustrated if you don’t immediately see something. Try again!

• If you don’t see anything at all, it’s probably because the specimen is not

positioned precisely beneath the objective lens. Carefully move the slide on the stage

in order to bring the specimen into the correct position.

WARNING!

At the highest magnification (600x), the

objective is so long that you have to be careful not

to let it hit the slide!





…





Always start with the lowest

magnification in order to orient

yourself with an overview of the

object on the slide.

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