Thames & Kosmos Kids First: Biology Lab (V 2.0) handleiding

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Thames & Kosmos

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Handleiding

Cells in Cross Section

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Pine needles,

sliced thin

You will need

– Slide

– Cover slip

– Pipette

– Razor blade

– Tweezers

– Dissecting needle

– Water

– Needle from pine tree

– Expanded polystyrene

foam piece

Here’s how

1. Prepare a slide. Make sure you have a sharp

razor blade that you have covered on one side

according to the instructions on page 10.

2. Cut a slit in the piece of expanded polystyrene

foam and insert the pine needle into the slit.

Cut very thin slices, as described on the

bottom of page 10.

3. Examine your pine needle slices and choose

the thinnest. Use the tweezers or dissecting

needle to carefully place this slice in the water

droplet on the slide.

WARNING!

The razor blade

is very sharp — and it needs

to be!

EXPERIMENT

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In biology, tissue is a group

of similar cells. You can see

a variety of tissue types in

the pine needle slices even

without using a dye. The

supportive wall tissue

provides a stable structure

for leaves and stalks. Inside

the walls, there are tube-

like passageways for water

and nutrients, typically

also surrounded by

supportive tissue. At the

very edge, you can easily

see the outer cuticle

covering, composed of lots

of small cells.

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You can tell right off the bat if a slice is too

thick by the fact that the cover slip will not

lie flat on the slide. You can only tell by

looking through the microscope if a slice

really is thin enough.

Ivy shoot

cross section

Corn stalk

cross section

Pine needles

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