Thames & Kosmos Genetics & DNA handleiding

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Thames & Kosmos

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Handleiding

The DNA

becomes visible

YOU WILL NEED

→ cold denatured alcohol in

brown bottle from

Experiment 

→ pipette

→ clear tomato-soap filtrate

from Experiment 

→ test tube stopper

This experiment is a cold bath for the

ﬁltrate, which is what researchers call the

clear solution resulting from ﬁltration.

HERES HOW

1. Get the denatured alcohol from the

freezer, take a small amount into the

pipette, and carefully let the cold

alcohol run down the wall of the tilted

test tube.

2. Repeat until the liquid level reaches the

second marker. In a real laboratory

report, it would say: Add an equal

quantity of denatured alcohol to the

ﬁltrate.

3. You will see that the denatured alcohol has

formed a layer above the ﬁltered tomato

liquid, because the alcohol is lighter than

water. You may even be able to see a few

clouds or ﬂakes at the place where the two

liquids meet. We want more of those.

4. Close the test tube with its stopper and

turn it several times, slowly and gently,

upside down and then right-side up again.

Continue until the spirits and tomato liquid

are mixed.

Do you see something in the test tube?

If everything has gone according to plan,

you should see some pale threads. If you

can’t see anything, put the test tube back

in the freezer. After a few hours, you will

see ﬁne white threads and ﬂakes in the

test tube: They are none other than the

fabled genetic material, or DNA for short.

You have isolated the code that makes

tomatoes come from tomatoes!

In the watery tomato solution, the

genetic material is ﬁnely dissolved and

thus evenly distributed and invisible to

us. But when the material is surrounded

by cold alcohol, it balls up and separates

from the liquid. This is similar to the way

that sour milk curdles when you pour it

into hot coffee.

→

WHATS HAPPENING







Isolating Genetic Material | 

EXPERIMENT 

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